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辣根过氧化物酶标记的羊抗兔IgG H&L
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产品编号bs-0295G-HRP英文名称Goat Anti-Rabbit IgG H&L / HRP中文名称辣根过氧化物酶标记的羊抗兔IgG H&L别 名Goat Anti-Rabbit IgG H&L (HRP); Immunoglobulin G; Specific References (703) | bs-0295G-HRP has been referenced in 703 publications.111 [IF=5.21] Ma, Yanqin, et al. "Inflammatory responses induced by fluoride and arsenic at toxic concentration in rabbit aorta." Archives of toxicology 86.6 (2012): 849-856. WB ; Rabbit. 222111 [IF=4.26] Wang, Ye-Jun, et al. "Two oral HBx vaccines delivered by live attenuated< i> Salmonella: Both eliciting effective anti-tumor immunity." Cancer letters 263.1 (2008): 67-76. WB ; Rabbit. 222111 [IF=4.26] Li, Gang, et al. "Overexpression of human papillomavirus (HPV) type 16 oncoproteins promotes angiogenesis< i> via enhancing HIF-1?? and VEGF expression in non-small cell lung cancer cells."Cancer letters 311.2 (2011): 160-170.. WB ; Rabbit. 222111 [IF=3.55] Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012). WB ; Rabbit. 222111 [IF=3.54] Zhang, Boce, Ariella Feldman, and Qin Wang. "A novel insight in rapid allergen detection in food systems: From threshold dose to real-world concentration."Sensors and Actuators B: Chemical?186 (2013): 597-602. ELISA ; Rabbit. 222111 [IF=3.06] Zhang, Zi?\ji, et al. "iRNA expression profile during osteogenic differentiation of human adipose?\derived stem cells."Journal of cellular biochemistry 113.3 (2012): 888-898..c WB ; Rabbit. 222111 [IF=3.06] Li, Guiying, et al. "Gremlin Aggravates Hyperglycemia?\Induced Podocyte Injury by a TGF??/Smad Dependent Signaling Pathway."ournal of cellular biochemistry?(2013).:: WB ; Rabbit. 222111 [IF=2.8] Jiang, An, et al. "miR-615-3p promotes the phagocytic capacity of splenic macrophages by targeting ligand-dependent nuclear receptor corepressor in cirrhosis-related portal hypertension." Experimental Biology and Medicine 236.6 (2011): 672-680. WB ; Rabbit. 222111 [IF=2.75] Dong, Xian-Zhe, et al. "A representative prescription for emotional disease, Ding-Zhi-Xiao-Wan restores 5-HT system deficit through interfering the synthesis and transshipment in chronic mild stress-induced depressive Rats." Journal of Ethnopharmacology ( WB ; Rabbit. 222111 [IF=2.7] Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013). WB ; Rabbit. 222111 [IF=2.7] WANG, Peng, et al. "Construction and identification of a recombinant adenovirus vector expressing His-tag-ICP47 fusion gene." Life Science Journal 9.1 (2012). Rabbit. 222111 [IF=2.58] Li, Yuping, et al. "Direct detection of rutin degrading isozymes with PAGE."Analytical Biochemistry (2013). WB ; Rabbit. 222PubMed:24050968111 [IF=2.41] Cong, Liang, et al. "Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic< i> hIGF-I silkworms." Biochemical and biophysical research communications 410.4 (2011): 721-725. WB ; Rabbit. 222111 [IF=2.07] Ma, Song‐Tao, et al. "Effect of Arctiin on Glomerular Filtration Barrier Damage in STZ‐Induced Diabetic Nephropathy Rats." Phytotherapy Research (2012). Rabbit. 222111 [IF=1.99] Fan, Tingjun, et al. "Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane." Molecular vision 17 (2011): 469. Rabbit. 222111 [IF=1.89] Yan, Shu, et al. "Expression of endoplasmic reticulum stress-related factors in the retinas of diabetic rats." Experimental diabetes research 2012 (2011). WB ; Rabbit. 222111 [IF=1.77] Chen, Cui-Ying, et al. "Characterization of cytotoxicity-related gene expression in response to virulent Marek’s disease virus infection in the bursa of Fabricius." Research in Veterinary Science (2012). WB ; Rabbit. 222111 [IF=1.74] Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013). WB ; Rabbit. 222111 [IF=.96] Yang, L., et al. "Transplantation of Schwann Cells Differentiated from Adipose-derived Stem Cells Modifies Reactive Gliosis after Contusion Brain Injury in Rats." Journal of International Medical Research 39.4 (2011): 1344-1357. WB ; Rabbit. 222111 [IF=.57] Lu, Shuang, et al. "Preparation and characterization of the polyclonal antibody against GAR domain of microtubule actin cross-linking factor 1 (MACF1)." Afr. J. Biotechnol 10 (2011): 2156-2162. ELISA ; Rabbit. 222111 [IF=.34] Feng, Yongqiang, et al. "Combination of ketamine and xylazine exacerbates cardiac dysfunction in severely scalded rats during the shock stage." Experimental and Therapeutic Medicine (2013). WB ; Rabbit. 222PubMed:4137240111 [IF=2.59] Zhang, Huizhen, et al. "Treatment with carnosine reduces hypoxia-ischemia brain damage in a neonatal rat model." European Journal of Pharmacology (2014). WB ; Rabbit. 222111 [IF=3.42] Xu, R., et al. "Secretoneurin Induces Airway Mucus Hypersecretion by Enhancing the Binding of EGF to NRP1." Cellular Physiology and Biochemistry 33.2 (2014): 446-456. WB ; Rabbit. 222111 [IF=3.01] Wang, Ying, et al. "Iso-suillin from the mushroom< i> Suillus flavus induces cell cycle arrest and apoptosis in K562 cell line." Food and Chemical Toxicology (2014). WB ; Rabbit. 222111 [IF=1.73] Zeng, Q. J., et al. "Decorin-induced proliferation of avian myoblasts involves the myostatin/Smad signaling pathway." Poultry Science 93.1 (2014): 138-146. WB ; Rabbit. 222111 [IF=3.73] Chen, Feng, et al. "Up-Regulating CYP3A4 Expression in C3A Cells by Transfection with a Novel Chimeric Regulator of hPXR-p53-AD." PLOS ONE 9.5 (2014): e95752. WB ; Rabbit. 222111 [IF=3.5] Zhang, Peng, et al. "In Situ Tracking the Intracellular Delivery of Antisense Oligonucleotides by Fluorescein Doped Silica Nanoparticles." Talanta (2014). WB ; Rabbit. 222111 [IF=7.6] Gao, Fuping, et al. "Cytotoxicity and therapeutic effect of irinotecan combined with selenium nanoparticles." Biomaterials (2014). IHC-P ; Rabbit. 222111 [IF=3.79] Luo, Xiaoming, et al. "Antimetastasis and antitumor efficacy promoted by sequential release of vascular disrupting and chemotherapeutic agents from electrospun fibers." International Journal of Pharmaceutics (2014). IHC-P ; Rabbit. 222111 [IF=3.39] Qiao, Hui, et al. "Progressive alterations of hippocampal CA3-CA1 synapses in an animal model of depression." Behavioural Brain Research (2014). WB ; Rabbit. 222抗体来源Goat克隆类型Polyclonal交叉反应Rabbit, 产品应用WB=1:10000-50000 ELISA=1:50000-200000 IHC-P=1:200 not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.性 状Liquid浓 度2.0 mg/ml免 疫 原Native rabbit IgG 亚 型IgG纯化方法affinity purified by Protein G, nonspecific adsorbed缓 冲 液10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.产品图片Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.Immobilized rabbit IgG (bs-0295P, nothing else) at 0.2μg/well can bind Goat Anti-Rabbit IgG H&L/HRP antibody (Cat. No. bs-0295G-HRP).
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