D(+)-Trehalose dihydrate;D-海藻糖二水产品简介CAS6138-23-4中文名称D-海藻糖二水英文名称D(+)-Trehalose dihydrate别名α,α-Trehalosedihydrate纯度HPLC≥98%单位瓶分子式C12H22O11·2H2O分子量378.33外观(性状)White to off-white Solid储存条件Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in WaterECEINECS 202-739-6MDLMFCD00071594SMILESO[C@H]1[C@@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)O[C@H](CO)[C@@H](O)[C@@H]1O.[2 H2O]描述D-(+)-Trehalose dihydrate可作赋形剂。(D-(+)-Trehalose dihydrate can be used as excipient.)靶点Others通路Others生物活性D-(+)-Trehalose dihydrate可用作食品成分和药物赋形剂。[1]In Vitro海藻糖二水合物是一种安全的,天然存在的二糖,用作食品成分和药物赋形剂[1]。数据来源文献[1]. Megarry AJ, et al. Amorphous trehalose dihydrate by cryogenic milling. Carbohydr Res. 2011 Jun 1;346(8):1061-4.规格20mg 10mM*1mL in Water 100mgD-(+)-Trehalose dihydrate可作赋形剂。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.D(+)-Trehalose dihydrate;D-海藻糖二水_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
D-Sorbitol D-山梨醇产品简介CAS50-70-4中文名称D-山梨醇英文名称D-Sorbitol别名D-山梨糖醇单位瓶纯度HPLC≥98%分子式C6H14O6 分子量182.17外观(性状)White to off-white Solid储存条件Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in Water/DMSOMDLMFCD00004708ECEINECS 200-061-5InChIKeyFBPFZTCFMRRESA-JGWLITMVSA-NInChIInChI=1S/C6H14O6/c7-1-3(9)5(11)6(12)4(10)2-8/h3-12H,1-2H2/t3-,4+,5-,6-/m1/s1PubChem CID5780SMILESOC[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O描述是蔷薇科植物的主要光合作用产物,是一种具有保湿功能的特殊甜味剂,可作为湿润剂、螯合剂和稳定剂。(It is the main photosynthesis product of Rosaceae, a special sweetener with moisturizing function, and can be used as wetting agent, chelating agent and stabilizer.)靶点Endogenous Metabolite通路Others规格100mg 10mM*1mL (in Water) 10mM*1mL(in DMSO) 200mg是蔷薇科植物的主要光合作用产物,是一种具有保湿功能的特殊甜味剂,可作为湿润剂、螯合剂和稳定剂。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.D-Sorbitol D-山梨醇_抑制剂&拮抗剂&激动剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
Hygromycin B 潮霉素B产品简介CAS31282-04-9中文名称潮霉素B英文名称Hygromycin B别名Destomycin A; Hygrovetin单位瓶纯度≥90%分子式C20H37N3O13 分子量527.52外观(性状)Off-white to yellow Solid储存条件Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in Water ≥100mg/mLMDLMFCD00036846ECEINECS 250-545-5InChIKeyGRRNUXAQVGOGFE-XKIAHZFYSA-NInChIInChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1PubChem CID23777801SMILESO[C@H]([C@H]1O)C2(O[C@@]([C@@H](N)CO)([H])[C@@H]1O)O[C@@]([C@@H](O[C@@H]3CO)O[C@@]([C@H]([C@H](N)C4)O)([H])[C@@H]([C@H]4NC)O)([H])[C@@]([C@H]3O)([H])O2描述是抑制原核和真核细胞的氨基糖苷类抗生素,通过干扰移位、促进70S核糖体的误译来抑制蛋白质合成。(It is an aminoglycoside antibiotic that inhibits prokaryotic and eukaryotic cells, inhibiting protein synthesis by interfering with translocation and promoting mistranslation of 70S ribosomes.)靶点Bacterial;Fungal通路Anti-infection生物活性Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.[1-3]In VitroHygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus[1]. Hygromycin B at 0.38 mM concentration completely halts yeast cell growth in rich media, presumably by preventing protein synthesis by cytoplasmic ribosomes. Polypeptide synthesis in cell-free extracts from rabbit reticulocytes, wheat germ and yeast is strongly blocked by low concentrations ofhygromycin B. The antibiotic inhibits peptide chain elongation by yeast polysomes by preventing elongation factor EF-2-dependent translocation. The inhibition of translocation by hygromycin B might result from the stabilization of peptidyl-tRNA bound to the ribosomal acceptor site[2].In VivoHygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading in vivo[3]. Constitutive expression of the bacterial hygR gene in transgenic mice in vivo confers resistance to hygromycin B[4].动物实验Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10 consecutive days[4].数据来源文献[1]. Pardo JM, et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus--the producing organism. J Gen Microbiol. 1985 Jun;131(6):1289-98.[2]. González A, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta. 1978 Dec 21;521(2):459-69.[3]. Pfister P, et al. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003 May;47(5):1496-502.规格200mg 10mM*1mL (in Water) 1g备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.
Posaconazole;泊沙康唑产品简介CAS171228-49-2中文名称泊沙康唑英文名称Posaconazole别名Pcz;Noxafil;Stereoisomer;Posconazole纯度HPLC≥98%单位支分子式C37H42F2N8O4 分子量700.78外观(性状)White to off-white Solid储存条件Powder : -20℃, 2 years;In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in DMSOECEINECS 200-659-6MDLMFCD00941162SMILESFC1=CC=C([C@@]2(CN3C=NC=N3)C[C@H](COC4=CC=C(N5CCN(C6=CC=C(N7C=NN([C@@H](CC)[C@H](C)O)C7=O)C=C6)CC5)C=C4)CO2)C(F)=C1描述Posaconazole 是一种广谱的,二代三唑类物质,具有抗真菌活性。(Posaconazole is a broad-spectrum, second-generation triazole with antifungal activity.)靶点Fungal通路Anti-infection生物活性Posaconazole 是一种广谱的,二代三唑类物质,具有抗真菌作用。[1-5]In VitroPosaconazole对念珠菌和曲霉菌的分离株具有活性。表现出对氟康唑,伏立康唑和两性霉素B的抗性,并且比其他三唑对抗接合菌的活性要高得多[2]。Posaconazole具有有效的杀螨活性。胺碘酮与Posaconazole协同作用。 Posaconazole也会影响和破坏T. cruzi中的Ca2 +稳态。Posaconazole阻断麦角甾醇的生物合成,麦角甾醇是寄生虫存活的必要条件。Posaconazole对epimastigote(细胞外)阶段的增殖具有明显的剂量依赖性作用,最小抑制浓度为20 nM,IC50为14 nM。针对临床相关的细胞内无鞭毛体形式的寄生虫,Posaconazole甚至更有效。Posaconazole的抑制浓度最低,IC50值分别为3 nM和0.25 nM [1]。In Vivo单独使用胺碘酮治疗感染动物可减少寄生虫血症,提高60天存活率(未治疗对照组为0%,胺碘酮治疗组为40%),与Posaconazole联合使用可延缓寄生虫血症的发生[1]。与在禁食状态下单独使用Posaconazole相比,Posaconazole和Boost Plus的共同给药增加了药物暴露。食物,尤其是脂肪含量高的膳食,可显著提高Posaconazole的生物利用度。当与高脂肪和无脂肪餐一起食用时,全身暴露于Posaconazole分别增加4倍和2.6倍[3]。Posaconazole和胺碘酮可构成有效的抗T。 cruzi疗法副作用小[4]。每日两次剂量≥15mg/ kg体重,Posaconazole可延长小鼠的存活时间并减轻组织负担[5]。细胞实验寄生虫的epimastigote形式在肝输注胰蛋白胨培养基中培养,其中补充有10%新出生的小牛血清,在28℃下进行强烈(120rpm)搅拌。培养物以2×106 epimastigotes mL-1的细胞密度开始,并且以0.5-1.0×107 epimastigotes mL-1的细胞密度添加药物。通过使用电子粒子计数器以及通过血细胞计数器直接计数来测量细胞密度。细胞活力随后是台盼蓝排除,使用光学显微镜检查。如前所述,无鞭毛体在Vero细胞中培养,所述Vero细胞维持在补充有1%胎牛血清的最小必需培养基中,在37℃下在潮湿气氛(95%空气-5%CO 2)中。每个细胞用10个组织培养物衍生的trypomastigotes感染细胞2小时,然后用磷酸盐缓冲盐水(PBS)洗涤三次以除去非粘附寄生虫。添加含有和不含药物的新鲜培养基,将细胞培养96小时,培养基更换为48小时。使用光学显微镜直接测定感染细胞的百分比和每个细胞的寄生虫数量,并如前所述进行结果的统计分析。使用GraFit程序通过非线性回归计算IC 50值。对照和药物处理的细胞外epimastigotes中的细胞质游离Ca 2+浓度通过荧光测定法使用Fura-2测定,再次如前所述。通过使用时间扫描共聚焦显微镜,在用克氏锥虫无鞭毛体感染的个体Vero细胞上监测亚细胞Ca 2+水平和线粒体膜电位,如其他地方详细描述的。简而言之,用T. cruzi无鞭毛体严重感染(72小时)的Vero细胞接种到22×40mm玻璃盖玻片(0.15mm厚度)上,并与10μM细胞渗透性Rhod-2和10μg/ mL罗丹明-123同时孵育。在37℃下在培养基中50分钟,然后洗涤并与含有或不含有胺碘酮的林格氏溶液一起温育。在所使用的条件下,Rhod-2的荧光主要来自细胞内富含Ca2 +的区室,如线粒体,因为其对Ca2 +的低亲和力限制了其在Vero细胞或无鞭毛体的Ca2 + - 细胞质中的荧光。罗丹明-123是一种线粒体特异性阳离子染料,严格根据其膜电位分布在线粒体内膜上。动物实验通过使用急性恰加斯病的鼠模型进行体内研究,其中雌性NMRI-IVIC小鼠(20-25g)用105或103个血流试验鞭毛体感染,并且在24小时后开始药物治疗。对于Posaconazole(30剂)和/或每隔一天50mg/kg对于胺碘酮(15剂),以20mg/kg/d连续30天给予治疗。阴性对照(即未治疗的动物)仅接受载体,而阳性对照用抗-T治疗。克唑子化合物,硝呋莫司,50mg/kg/d,30天。数据来源文献[1]. Benaim G, et al. Amiodarone has intrinsic anti-Trypanosoma cruzi activity and acts synergistically with posaconazole. J Med Chem. 2006 Feb 9;49(3):892-9.[2]. Sabatelli F, et al. In vitro activities of posaconazole, fluconazole, itraconazole, voriconazole, and amphotericin B against a large collection of clinically important molds and yeasts. Antimicrob Agents Chemother. 2006 Jun;50(6):2009-15.[3]. Sansone-Parsons A, et al. Effect of a nutritional supplement on posaconazole pharmacokinetics following oral administration to healthy volunteers. Antimicrob Agents Chemother. 2006 May;50(5):1881-3.[4]. Veiga-Santos P, et al. Effects of amiodarone and posaconazole on the growth and ultrastructure of Trypanosoma cruzi. Int J Antimicrob Agents. 2012 Jul;40(1):61-71.[5]. Sun QN, et al. In vivo activity of posaconazole against Mucor spp. in an immunosuppressed-mouse model. Antimicrob Agents Chemother. 2002 Jul;46(7):2310-2.规格5mg 10mM*1mL in DMSO 10mgPosaconazole 是一种广谱的,二代三唑类物质,具有抗真菌活性。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.Posaconazole;泊沙康唑_Anti-Fungal_Anti-infection/抗感染_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
L-Glycine;L-甘氨酸产品简介CAS56-40-6中文名称L-甘氨酸英文名称L-Glycine别名H-Gly-OH纯度HPLC≥98%单位瓶分子式C2H5NO2分子量75.07外观(性状)White to off-white Solid储存条件Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in Water ≥ 6mg/mLECEINECS 200-272-2MDLMFCD00008131InChIKeyDHMQDGOQFOQNFH-UHFFFAOYSA-NInChIInChI=1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)PubChem CID750SMILESNCC(O)=O描述是中枢神经系统的一种抑制性神经递质,同时也是glutamate的一种联合激动剂,有促进谷氨酸能NMDA (N-methyl-D-aspartic acid ) 受体兴奋的潜能。(It is an inhibitory neurotransmitter in the central nervous system and a combined agonist of glutamate, which has the potential to promote the excitation of glutamatergic NMDA (N-methyl-D-aspartic acid) receptors.)靶点Others通路Others规格500mg 1g 10mM*1mL in Water备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.L-Glycine;L-甘氨酸_氨基酸及其衍生物_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇10000产品简介CAS25322-68-3中文名称聚乙二醇10000英文名称PEG10000单位瓶分子式HO(CH2CH2O)nH分子量10000外观(性状)Solid储存条件RT,2 years溶解性Soluble in WaterMDLMFCD00081839ECEINECS 203-473-3SMILES---描述聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。(Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on.)靶点---规格100g聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇10000_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇800产品简介CAS25322-68-3中文名称聚乙二醇800英文名称PEG800单位瓶分子式HO(CH2CH2O)nH分子量800外观(性状)White to off-white Solid储存条件RT,2 years溶解性Soluble in Water ≥100mg/mLMDLMFCD00081839ECEINECS 203-473-3SMILES---描述聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。(Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on.)靶点---规格100g聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇800_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇200产品简介CAS25322-68-3中文名称聚乙二醇200英文名称PEG200单位瓶分子式HO(CH2CH2O)nH分子量200外观(性状)Liquid储存条件RT,2 yearsMDLMFCD00081839ECEINECS 203-473-3SMILES---描述聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。(Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on.)靶点---规格100ml聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇200_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇20000产品简介CAS25322-68-3中文名称聚乙二醇20000英文名称PEG20000单位瓶分子式HO(CH2CH2O)nH分子量20000外观(性状)White to off-white Solid储存条件RT,2 years溶解性Soluble in Water ≥100mg/mLMDLMFCD00081839ECEINECS 203-473-3SMILES---描述聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。(Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on.)靶点---规格100g聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇20000_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇1500产品简介CAS25322-68-3中文名称聚乙二醇1500英文名称PEG1500单位瓶分子式HO(CH2CH2O)nH分子量1500外观(性状)Solid储存条件RT,2 years溶解性Soluble in WaterMDLMFCD00081839ECEINECS 203-473-3SMILES---描述聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。(Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on.)靶点---规格100g聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇1500_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇1000产品简介CAS25322-68-3中文名称聚乙二醇1000英文名称PEG1000单位瓶分子式HO(CH2CH2O)nH分子量1000外观(性状)White to off-white Solid储存条件RT,2 years溶解性Soluble in Water ≥100mg/mLMDLMFCD00081839ECEINECS 203-473-3SMILES---描述聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。(Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on.)靶点---规格100g聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇1000_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇4000产品简介CAS25322-68-3中文名称聚乙二醇4000英文名称PEG4000单位瓶分子式HO(CH2CH2O)nH分子量4000外观(性状)White to off-white Solid储存条件RT,2 years溶解性Soluble in Water ≥100mg/mLMDLMFCD00081839ECEINECS 203-473-3SMILES---描述聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。(Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on.)靶点---规格100g聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇4000_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇6000产品简介CAS25322-68-3中文名称聚乙二醇6000英文名称PEG6000单位瓶分子式HO(CH2CH2O)nH分子量6000外观(性状)White to off-white Solid储存条件RT,2 years溶解性Soluble in Water ≥100mg/mLMDLMFCD00081839ECEINECS 203-473-3SMILES---描述聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。(Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on.)靶点---规格100g聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇6000_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
PEG400;聚乙二醇400产品简介CAS25322-68-3中文名称聚乙二醇400英文名称PEG400别名聚环氧乙烷;聚氧乙烯400;氧化聚乙烯单位瓶分子式HO(CH2CH2O)nH分子量≈400外观(性状)Colorless Liquid储存条件RT,2 yearsMDLMFCD00081839ECEINECS 203-473-3SMILESOCCCCOC[H].[n]描述是可用于多种药物制剂的常用助溶剂。(It is a commonly used cosolvent for a variety of pharmaceutical preparations.)规格100ml是可用于多种药物制剂的常用助溶剂。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.PEG400;聚乙二醇400_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇8000产品简介CAS25322-68-3中文名称聚乙二醇8000英文名称PEG8000单位瓶分子式HO(CH2CH2O)nH分子量8000外观(性状)White to off-white Solid储存条件RT,2 years溶解性Soluble in Water ≥100mg/mLMDLMFCD00081839ECEINECS 203-473-3SMILES---描述聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。(Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on.)靶点---规格100g聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇8000_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇3350产品简介CAS25322-68-3中文名称聚乙二醇3350英文名称PEG3350单位瓶分子式HO(CH2CH2O)nH分子量3350外观(性状)White to off-white Solid储存条件RT,2 years溶解性Soluble in Water ≥100mg/mLMDLMFCD00081839ECEINECS 203-473-3SMILES---描述聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。(Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on.)靶点---规格100g聚乙二醇是一种高分子聚合物,可用于药物缓释和固定化酶的载体、医用高分子材料表面改性等多种方面的研究。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇3350_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
聚乙二醇300产品简介CAS25322-68-3中文名称聚乙二醇300英文名称PEG300别名聚环氧乙烷;氧化聚乙烯单位瓶分子式HO(CH2CH2O)nH分子量≈300外观(性状)Colorless Liquid储存条件RT,2 yearsMDLMFCD00081839ECEINECS 203-473-3SMILES---描述PEG300 是一种中性的生物相容性亲水聚合物。(PEG300 is a neutral biocompatible hydrophilic polymer.)靶点---规格100mlPEG300 是一种中性的生物相容性亲水聚合物。常作为药物载体、助溶剂等使用。使用浓度(仅供参考)20mg/kg 雷帕霉素,2%DMSO+30%PEG300+5%Tween80+63%ddH2O10 mg/kg Liproxstatin-1,2% DMSO + 40% PEG300 + 2%Tween80 + ddH2O30 mg/kg STF -083010,2% DMSO + 40% PEG300 + 5% Tween 80 + ddH2O 7 mg/mL Mdivi-1,5% DMSO、40% PEG300、5% Tween 80 + ddH2O 5 mg/mL SAHA, 2% DMSO+30% PEG300 + ddH2O备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.聚乙二醇300_助溶剂&增溶剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
Gentamicin sulfate;硫酸庆大霉素产品简介CAS1405-41-0中文名称硫酸庆大霉素英文名称Gentamicin sulfate别名庆大霉素硫酸酯;庆大霉素硫酸酶活/效价≥590IU/mg纯度≥590IU/mg单位瓶分子式C21H43N5O7·H2SO4 分子量575.67外观(性状)White to off-white Solid储存条件Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in WaterECEINECS 215-778-9MDLMFCD00270181SMILESO=S(O)(O)=O.O[C@]1(C)C(NC)[C@@H](O)[C@@H](O[C@H]2C(N)C[C@H](N)[C@@H](O[C@@H]3[C@H](N)CC[C@@H]([R])O3)[C@@H]2O)OC1.NC[*].C[C@H]([*])N.C[C@H]([*])NC.[R=].[or].[or]描述硫酸庆大霉素为氨基糖苷类抗生素。对各种革兰阴性细菌及革兰阳性细菌都有良好的抗菌作用,作用机制是与细菌核糖体30S亚单位结合,抑制细菌蛋白质的合成(Gentamicin sulfate is an aminoglycoside antibiotic. It has good antibacterial effect on various gram-negative bacteria and gram-positive bacteria. The mechanism of action is to bind to the 30S subunit of bacterial ribosome to inhibit the synthesis of bacterial protein.)靶点Bacterial通路Anti-infection生物活性Gentamicin sulfate是一种氨基糖苷类抗生素,抑制革兰氏阳性菌和革兰氏阴性细菌的生长并可抑制组织培养中的多种支原体菌株。抑制DNase I活性的IC50值为0.57 mM。[1-4]IC500.57 mM (DNase I)[1]In Vitro庆大霉素是一种比联合青霉素 - 链霉素更有效的体外细菌抑制剂,对组织培养单层无毒,并且不抑制病毒复制。它已被成功地用作商业真菌学培养基中的添加剂以抑制细菌的生长,并且已被证明对于更广泛的生物(铜绿假单胞菌,变形杆菌和粪链球菌)比青霉素和链霉素具有杀菌作用。它不会干扰组织培养中某些埃可病毒和脊髓灰质炎病毒产生的细胞病变效应,对恒河猴肾,HeLa和人羊膜细胞无毒,并且在高压灭菌温度下稳定[2]。庆大霉素是由小单孢菌属(Micromonospora)的各种物种产生的。商品庆大霉素主要由不同的庆大霉素C组分组成。 Yoshizawa阐明了与A位点RNA结合的庆大霉素C1a的3D结构。庆大霉素C1a结合在RNA的A位点的主要沟中[3]。In Vivo庆大霉素目前是用于治疗严重感染的首选氨基糖苷类,替代品为阿米卡星,奈替米星和妥布霉素。庆大霉素制剂可以三种形式商购,即耳,眼和局部,基于治疗耳道,眼睛和皮肤感染的相应功能。发现口服和注射形式的庆大霉素对鼠疫耶尔森氏菌具有有效的抗菌活性,如小鼠感染模型所示[3]。用多达9剂甲氧西林或庆大霉素治疗显示异物上的细菌显著减少[4] 。动物实验小鼠:细菌攻击小鼠用甲氧西林,庆大霉素,甲氧西林和庆大霉素,或不用抗生素治疗。治疗每天给药三次,持续3天。每剂甲氧西林是每只小鼠75mg(3g/kg体重),并且庆大霉素剂量是每只小鼠0.75mg(0.03g/kg)。在0.1或0.5mL盐水中皮下给予抗生素。处死小鼠并收集血清和抽吸样品[4]。数据来源文献[1]. Xu W, et al. A rapid and sensitive method for kinetic study and activity assay of DNase I in vitro based on a GO-quenched hairpin probe. Anal Bioanal Chem. 2016 May;408(14):3801-9.[2]. Rudin A, et al. Antibacterial activity of gentamicin sulfate in tissue culture. Appl Microbiol. 1970 Dec;20(6):989-90.[3]. Kumar CG, et al. Microbial biosynthesis and applications of gentamicin: a critical appraisal.[4]. Espersen F, et al. Effect of treatment with methicillin and gentamicin in a new experimental mouse model of foreignbody infection. Antimicrob Agents Chemother. 1994 Sep;38(9):2047-53.规格100mg 10mM*1mL in Water 500mg硫酸庆大霉素为氨基糖苷类抗生素。对各种革兰阴性细菌及革兰阳性细菌都有良好的抗菌作用,作用机制是与细菌核糖体30S亚单位结合,抑制细菌蛋白质的合成备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.Gentamicin sulfate;硫酸庆大霉素_Anti-Bacterial_Anti-infection/抗感染_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
牛血清白蛋白Ⅴ产品简介CAS9048-46-8中文名称牛血清白蛋白Ⅴ英文名称Bovine Serum Albumin别名BSA, 牛白纯度HPLC≥98%单位支分子量≈66kDa外观(性状)White to yellow Solid储存条件Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in WaterMDLMFCD00131855ECEINECS 232-936-2InChIKeyQYSGYZVSCZSLHT-UHFFFAOYSA-NInChIInChI=1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11PubChem CID6432描述是牛血清中的一种球蛋白,可用于多种生物化学实验。一般做为稳定剂被用于限制酶或者修饰酶的保存溶液和反应液中,还能防止酶吸附到管壁而损失。在PCR体系中有助于Taq酶的稳定性及活性,可以提高PCR的效率(It is a globulin in bovine serum and can be used in a variety of biochemical experiments. It is generally used as a stabilizer in the storage solution and reaction solution of restriction enzymes or modified enzymes, and can also prevent enzymes from being adsorbed to the tube wall and lost. Contributes to the stability and activity of Taq enzyme in the PCR system, and can improve the efficiency of PCR)靶点Others通路Others生物活性Bovine Serum Albumin (BSA) 是由三个同源全 α 结构域组成的 583 个氨基酸的蛋白质。BSA 是一种球状蛋白,可用于多种生物化学实验。[1]In Vitro牛血清白蛋白(BSA)是由三个同源的全α结构域组成的583残基蛋白,以心形结构组织。牛血清白蛋白构成ca.所有血浆蛋白的60%并且结合并运输大量生理和非生理配体。牛血清白蛋白含有17个二硫键和一个未配对的半胱氨酸(Cys34),其促进二聚化并且还影响高级缔合,因为如果Cys34与另一种化合物共价结合,则聚集速率减慢。用于原纤维细胞毒性的MTT测定显示牛血清白蛋白有利于细胞生长,而不管其聚集状态。通过膜透化测定证实缺乏细胞毒性。在随后的40小时中,观察到处理细胞的存活率增加300-400%,表明与牛血清白蛋白一起孵育的细胞比未处理的细胞具有更高的存活率[1]。细胞实验在96孔微量培养板中制备样品,并且所有不含样品的孔用200μLPBS填充以使蒸发最小化。向每个孔加载100μLSH-SY5Y生长培养基,含或不含10.000 SH-SY5Y神经母细胞瘤细胞。通过添加25μL缓冲液开始测定,其中有或没有5mg/mL超声处理的牛血清白蛋白,其预先在70℃温育0,2或96小时(给出最终BSA浓度为1mg/mL)。将所有样品组合装入三联体中,并且对于六种牛血清白蛋白组合中的每一种,加载相应的三联体而不含细胞,用作空白。一个三联体也没有细胞和BSA,只有一个没有牛血清白蛋白[1]。数据来源文献[1]. Holm NK, et al. Aggregation and fibrillation of bovine serum albumin. Biochim Biophys Acta. 2007 Sep;1774(9):1128-38规格200mg 1g是牛血清中的一种球蛋白,可用于多种生物化学实验。一般做为稳定剂被用于限制酶或者修饰酶的保存溶液和反应液中,还能防止酶吸附到管壁而损失。在PCR体系中有助于Taq酶的稳定性及活性,可以提高PCR的效率备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.牛血清白蛋白Ⅴ_Others_Others_抑制剂&拮抗剂&激动剂_小分子化合物_索莱宝-专业生化试剂网上商城 (solarbio.com)
Puromycin Dihydrochloride ;嘌呤霉素二盐酸盐产品简介CAS58-58-2中文名称嘌呤霉素二盐酸盐英文名称Puromycin dihydrochloride别名二氢氯化嘌呤毒素;Stillomycin纯度HPLC≥98%单位支分子式C22H31Cl2N7O5分子量544.43外观(性状)White to light yellow Solid储存条件Powder : -20℃, 2 years;In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in WaterECEINECS 200-387-8MDLMFCD00012691SMILESOC[C@@H]1[C@@H](NC([C@@H](N)CC2=CC=C(OC)C=C2)=O)[C@H]([C@H](N3C=NC4=C3N=CN=C4N(C)C)O1)O.[H]Cl.[H]Cl描述Puromycin是一种抑制 protein synthesis 的氨基糖苷类抗生素。(Puromycin is an aminoglycoside antibiotic that inhibits protein synthesis.)靶点Bacterial通路Anti-infection生物活性Puromycin dihydrochloride是嘌呤霉素的二盐酸盐。 Puromycin是一种抑制 protein synthesis 的氨基糖苷类抗生素。[1-3]In Vitro嘌呤霉素在氨酰基-sRNA形成后阻断蛋白质合成,同时它导致小肽的积累。这两种效应似乎都是由于核糖体结合的肽基-sRNA的分裂,导致不完整肽链的释放[1]。嘌呤霉素是氨酰基-tRNA的3末端的类似物,通过与生长的多肽链非特异性连接而导致翻译过早终止。嘌呤霉素具有两种抑制作用模式。第一种是通过作为受体底物,其攻击P位点中的肽基-tRNA以形成新生肽。第二种是通过与氨酰基-tRNA竞争结合A位点[2]。当以最小量使用时,嘌呤霉素掺入新合成的蛋白质直接反映体外mRNA翻译的速率。嘌呤霉素免疫检测是放射性氨基酸标记的有利替代方案。它允许通过免疫荧光显微镜直接评估单细胞中的翻译活性,并通过荧光激活细胞分选直接评估异质细胞群[3]。数据来源文献[1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. 1964 Apr;51:585-92.[2]. Miyamoto-Sato E, et al. Specific bonding of puromycin to full-length protein at the C-terminus. Nucleic Acids Res. 2000 Mar 1;28(5):1176-82.[3]. Schmidt EK, et al. SUnSET, a nonradioactive method to monitor protein synthesis. Nat Methods. 2009 Apr;6(4):275-7规格5mg 10mM*1mL in Water 10mg 25mgPuromycin是一种抑制 protein synthesis 的氨基糖苷类抗生素。使用本产品的应用案例(仅供参考)In VitroCell(OC cells,2 μg/mL puromycin)PCNP expression plasmid and empty vector, the PCNP shRNA (sh-PCNP group) and scramble shRNA (sh-Scb group) and were transfected into OC cells with Lipofectamine 3000 Transfection Reagent to construct stable cell lines. They were screened, respectively, by G418 (Solarbio, Shanghai, China) at a concentration of 800 μg/mL and puromycin (Solarbio, Shanghai, China) at a concentration of 2 μg/mL. 来源文献:Dong P, Fu H, Chen L, Zhang S, Zhang X, Li H, Wu D, Ji X. PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition. J Cell Mol Med. 2020 Jul;24(14):8221-8235. doi: 10.1111/jcmm.15491. Epub 2020 Jun 16. PMID: 32548978; PMCID: PMC7348179.备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.