非预染可曝光蛋白Marker ECL Protein MarkerNon pre-stained exposure protein Marker; Non pre-stained E-protein Marker; Non Prestained exposed protein Marker; Protein Ladder; Non pre dyed exposed Protein Marker; 电化学发光蛋白Marker; 非预染型可曝光蛋白Marker; 蛋白标准品; 蛋白分子量标准; 蛋白标准品(Marker); 可曝光marker; 产品简介:非预染可曝光蛋白Marker包括6条带,从14KD到150KD(条带分子量:14、20、40、60、90、150 kDa)。蛋白Marker中每一条带都能和抗体结合,能识别小鼠,兔,羊IgG(其他物种待测试更新),能精确指示目的条带位置,与一抗,二抗同时孵育即可。此款可曝光蛋白marker可搭配预染Marker一起使用。本产品能够应用于化学发光,荧光等检测。使用方法:1. 低温取出后,室温平衡至完全溶解,混匀即用,无需加热,无需再添加任何试剂。2. 本产品短时间内可以4℃保存,长时间保存请存放-20℃,有效期1年。3. 每次电泳Marker上样量3-5ul,溴酚蓝跑到凝胶底部结束电泳。4. 湿转膜或者半干法转膜,确保充分转膜,然后进行后续的封闭,孵育抗体和检测。5. 实验前请优先进行预实验验证可曝光Marker的使用体积,本产品会因不同抗体来源,抗体浓度以及抗体稀释液而不同。问题和解决方案:1. 条带较强可能原因1:是Marker使用量较多;解决方案:减少曝光Marker上样量。可能原因2:曝光时间过长;解决方案:缩短曝光时间,或改为5%脱脂奶粉封闭、稀释一抗。可能原因3:抗体浓度较高;解决方案:进一步稀释一抗。2. 条带较弱可能原因:封闭液和一抗来源;解决方案:适当增加可曝光marker的上样量。3. 条带缺失可能原因1:一抗为小鼠单抗且3%BSA封闭并稀释一抗;解决方案:更换成5%脱脂奶粉封闭、稀释一抗可能原因2:BSA浓度过高;解决方案:BSA浓度不高于3%,尽量使用5%脱脂奶粉封闭、稀释一抗。注意事项:一抗为小鼠单抗时,尽量避免使用BSA封闭、稀释一抗。
产品编号PV-0023英文名称 Polink-2 plus®Polymer HRP Detection System (Rabbit)中文名称超敏二步法免疫组化检测试剂盒(兔)别 名 SP-0023; Histostain-SP Kit; Histostain-SP (Streptavidin-Peroxidase)kit; 免疫组化检测试剂盒(兔); 超敏二步法免疫组化检测试剂盒(兔源一抗); 免疫组化染色试剂盒;保存条件 Store at 4 °C for one year.注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍 Polink-2 plus®是美国GBI公司推出的系列超敏二步法免疫组化检测试剂,它将二抗抗体分子的单价Fab片段和酶聚合在一起,替代传统方法中的二抗和三抗,直接放大抗原抗体结合的信号,既保留了抗体特异性结合抗原的能力,又可有效的避免聚合分子过大而造成的空间位阻。 本产品可用于检测兔源一抗,与传统的SP三步法相比,此试剂盒具有简单、快速、敏感等特点,因为系统中不再使用生物素,所以避免了由于内源性生物素所造成的背景染色。 本品独特的Polymer Helper有利于大分子的检测系统更好的结合所检测的一抗分子,此检测系统是目前已知的灵敏度最高的系列免疫组化检测系统。此系统比生物素-链酶卵白素的检测系统灵敏度提高数倍,所以应适当调整一抗的使用浓度。
S0134 Alcian Blue Stain Kit (pH 2.5) 阿利新蓝染色试剂盒(pH2.5)阿利新蓝染色液(pH2.5);标准阿利新蓝染色液(试剂盒)PH=2.5; 爱先蓝(PH2.5)染色液; 阿尔辛蓝染色液; 4℃避光保存,有效期12个月。产品介绍: 阿利新蓝(Alcian)又称爱先蓝或阿尔辛蓝等,是一种类铜钛花青共轭染料,最初用于纺织纤维染色。这种阳离子染料与酸性基团结合,也即阿尔辛蓝与组织内含有的阴离子基团如羧基和硫酸根形成不溶性复合物。 阿利新蓝由中央含铜的酞菁环与四个异硫脲基通过硫醚键相连而成,该异硫脲基呈中度碱性,使阿利新蓝带阳离子。pH值为2.5时,组织内的羧基电离,带有一个负电荷,与阿利新蓝中的阳离子形成盐键,使带有羧基的酸性黏液物质(硫酸黏蛋白和唾液黏蛋白)染色。pH值为1.0时,组织内的硫酸根电离,带有一个负电荷,与阿利新蓝中的阳离子形成盐键,使带有硫酸根的组织(如硫酸黏液物质)染色。 阿利新蓝染色液(pH2.5)又称标准阿利新蓝染色液,利用染液的不同pH 值可区分粘液物质的类属,pH为1时,羧基(COOH)不着色,硫酸基(OSO3H)着色,pH为 2.5 时,羧基染色良好而硫酸粘液着色不佳,使硫酸黏蛋白和唾液黏蛋白着色。中性黏蛋白(如胃黏膜和Brunner腺体部位的中性黏蛋白)不能与阿利新蓝反应。 本产品仅用于科研领域,不用于临床诊断。
产品编号S0180英文名称Masson-Fontana melanin stain kit中文名称Masson-Fontana黑色素染色试剂盒别 名Fontana-Masson Stain Kit; Masson-Fontana黑色素染色液;保存条件4℃避光保存,有效期3个月。产品介绍黑色素属于非血源性内生色素,是一组由黑色素母细胞产生的颜色从浅棕色到黑色的色素。这种色素通常出现在皮肤表皮、眼睛、大脑的黑质和毛囊中。黑色素有一个显著的物理性质,即完全不溶解于大多数有机溶剂——几乎可以肯定是由于黑素体中已形成的黑色素可与蛋白质紧密结合。黑色素另一个物理性质是能够被强氧化剂漂白,尽管这个过程是缓慢的。在病理情况下,这种色素也可出现在良性痣细胞瘤中和恶性黑色素瘤中。在常规HE染色中不呈黑色而是呈棕黄色或棕黑色。许多方法可用于识别黑色素和黑色素生成细胞,如还原方法:如Masson-Fontana银技术和Schmorl三价铁-铁氰化钾还原实验;酶方法:如多巴反应;荧光方法;免疫组织化学方法等。Masson-Fontana黑色素染色利用黑色素具有将银氨溶液还原为金属银特性即嗜银反应原理来显示黑色素,染色后黑色素呈黑色,该法属于常用的黑色素特殊染色法,效果较其他染色理想。本产品仅用于科研领域,不用于临床诊断。
S0197 Nissl stain kit (methyl violet method) 尼氏染色试剂盒(甲基紫法) 室温避光保存,有效期12个月。神经元细胞体包括一个具有皱褶核膜的大细胞核、稀疏的染色质和一个明显的核仁。在细胞体中细胞质是尼氏颗粒,即能够代表粗面内质网并在很多神经元中产生特异的斑点状嗜碱性表现的嗜碱性颗粒。尼氏颗粒可以用很多染色来显示如中性红、亚甲基蓝、甲苯胺蓝和甲基紫等。染色的变异、pH和分化的时间使一些染色既可以仅突出尼氏物质,也可以显示神经元的细胞核和神经胶质。尼氏体(Nissl body)或称尼氏小体是分布于神经细胞胞质内的三角形或椭圆形小块状物质,能被碱性染料如硫堇、亚甲蓝、甲苯胺蓝和焦油紫等染料染成紫蓝色。各种神经细胞内都含有尼氏体,但其形状、数量、分布位置常常不同。尼氏体也存在于树突中,但不在于轴突和包体的轴丘。尼氏体会因为生理状态的变化而变化,尼氏体是神经元内合成蛋白质合成的重要部位,当神经元受到刺激后,包体内的尼氏体会明显减少。 尼氏染色试剂盒(甲基紫法),也称Nissl Stain kit (Methyl violet method),主要特点是操作简便、染色稳定、分化时间短,适用范围广,可以用于石蜡组织切片的尼氏物质、神经元等的染色,尼氏体的存在和消失是神经细胞是否受损的重要指标,当发生脑炎、脑缺血、轴突反应等情况时,尼氏体会发生溶解甚至消失。 本产品仅用于科研领域,不用于临床诊断。
产品编号S0205英文名称Oil red O stain kit (for cell culture)中文名称油红O染色试剂盒(细胞专用)别 名 油红O染色液(培养细胞专用); 油红O染色液(细胞专用); 脂肪染色;保存条件4℃保存,有效期12个月。产品介绍脂质 (Lipid) 是中性脂肪、类脂及其衍生物的总称,其共同的物理特性是不溶于水,易溶于有机溶剂(如乙醇、乙醚等)。人体的脂肪主要有两种1、储存脂肪,如中性脂肪,主要分布于皮下、肾、胰腺等部位。2、结构脂肪,如类脂 (磷脂、糖脂、胆固醇等),主要分布于细胞内。中性脂肪 (Neutral fat) 是由三分子脂肪酸和一分子甘油组成的脂类,呈中性。中性脂肪染色经常采用苏丹Ⅱ、苏丹Ⅲ、苏丹Ⅳ、苏丹黑B、油红O法等。传统方法采用苏丹染料,最近发现偶氮染料油红O更适合脂肪的染色。油红O是很强的脂溶剂和染脂剂,较易与甘油三脂结合呈小脂滴状,与磷脂结合力稍差,其染色原理一般认为是物理上的溶液作用或吸附作用,借溶液作用使脂肪染色。 油红O染色试剂盒 (细胞专用) 主要用于显示人工培养细胞的脂肪变性和类脂质的异常沉着,细胞内出现多数中性脂肪滴,鉴别培养细胞中所发生的变化及其性质。标本不采用含有乙醇的固定液,脂肪的阳性染色结果呈橘黄至红色,但具体颜色因脂质浓度而定。 本产品仅用于科研领域,不用于临床诊断。
产品编号 S0255英文名称 Ruthenium red staining solution中文名称 钌红染色液保存条件 室温避光保存,有效期12个月。产品介绍美钌红(Ruthenium Red)分子式为Ru3O2Cl6.14(NH3),CAS号为11103-72-3,分子量为786.35,外观为红色晶体,可用做电压敏感的钙离子通道抑制剂。 钌红染色液在植物病虫害组织切品染色中,可将宿主植物组织中菌丝体和孢子染成红色。 本产品仅用于科研领域,不用于临床诊断。
产品编号 S0278英文名称 India ink中文名称 印度墨汁保存条件 室温避光保存,有效期12个月。产品介绍墨汁荚膜染色对于观察新型隐球菌有很好的作用,印度墨汁(India Ink)属于非常优质的墨汁,新型隐球菌菌体周围存在粘多糖荚膜物,用印度墨汁染色法能证明荚膜的存在。染色后新型隐球菌的荚膜取代了墨汁中的胶状碳粒,菌体被清楚无色透明的晕圈环所绕着。 本产品仅用于科研领域,不用于临床诊断。
产品编号S0286英文名称Crystal violet aqueous solution (1%)中文名称结晶紫染色液(1%)别 名Crystal Violet Staining Solution; 结晶紫水溶液(1%); 草酸铵结晶紫染色液(结晶紫染色液)(1%); 甲紫; 龙胆紫; 结晶紫;保存条件室温保存,有效期12个月。产品介绍结晶紫 (Crystal violet) 又称甲紫,分子式:C25H30N3Cl·9H2O,分子量:407.98,CAS:548-62-9。结晶紫属于碱性染料,能溶于水、乙醇,可以把组织和细胞核染成紫色。结晶紫染色液(Crystal Violet Staining Solution)在细胞学、组织学和细菌学等方面应用极广,是一种优良的染色剂。 本产品仅用于科研领域,不用于临床诊断。
产品编号Y-0044英文名称Amyloid Beta (25-35) Peptide中文名称β淀粉样肽25-35别 名Amyloid beta Peptide (25-35); Beta-Amyloid (25-35); Amyloid beta-Protein Fragment 25-35; A beta 25-35; Beta Amyloid 25-35. Aβ淀粉样肽25-35; β淀粉样肽(25-35); Amyloid β (25-35); Aβ 25-35; β Amyloid (25-35); β Amyloid 25-35.理论分子量1.060kDa性 状Lyophilized powder序 列GSNKGAIIGLM纯化方法HPLC活性Yes保存条件Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles.产品介绍Aβ是老年性痴呆发病的始动因子,并证实引起毒性主要部位在第25-35氨基酸之间(Aβ25-35) ,Aβ25-35(Aβ25-35 peptide)具有神经毒性,可使海马和隔区神经元的存活率下降,继而发生神经元凋亡的形态学改变.
产品编号D21062英文名称Levofloxacin中文名称左氧氟沙星别 名LVFX; (-)9-氟-2,3-二氢-3-甲基-10-(4-甲基-1-哌嗪基)-7-氧-7H-吡啶骈[1,2,3-DE]-[1,4]苯骈噁嗪-6-羧酸; 可乐必妥; 洛氟沙星; 左旋氧氟沙星; 利复星;保存条件Store at RT.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍CAS:100986-85-4分子式:C18H20FN3O4分子量:361.37MDL:MFCD07772024级别:BR纯度:≥98%(HPLC)熔点:218℃沸点:571.5℃ at 760 mmHg外观:白色至淡黄色粉末溶解性:较易溶于氯仿或丙酮,微溶于乙醇,乙醚或甲醇,几不溶于水。微溶于水或甲醇。溶于冰醋酸或二氯甲烷。敏感性:光敏感用途:为全合成抗菌素,治疗各种细菌感染。
Recombinant human IL-6 protein, C-His产品编号bs-41177P英文名称Recombinant human IL-6 protein, C-His中文名称重组人白介素6蛋白别 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6. ;Recombinant human IL-6 protein, C-His Specific References (1) | bs-41177P has been referenced in 1 publications.[IF=4.966] 222Xiaolong Zhang. et al. Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. Cancer Sci. 2020 Oct; 111(10): 3802–3812 other ; PubMed:32691974理论分子量15kDa检测分子量21 kDa性 状Lyophilized or Liquid浓 度>0.5 mg/ml物 种Human序 列30-212/212纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素Not analyzed表达系统E.coli活性Not tested标签C-His缓 冲 液20 mM Tris-HCl (pH=8.0) with 150 mM NaCl保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacterium Streptococcus pneumoniae.SWISS:P05231Gene ID:3569产品图片
Recombinant mouse IL-6 protein, C-His产品编号bs-10861P英文名称Recombinant mouse IL-6 protein, C-His中文名称重组小鼠白介素IL-6蛋白别 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6; Recombinant mouse IL-6 protein, C-His 理论分子量23kDa检测分子量26 kDa性 状Lyophilized or Liquid浓 度>0.5 mg/ml物 种Mouse序 列25-211/211纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素Not analyzed表达系统E.coli活性Not tested标签C-His缓 冲 液20 mM Tris-HCl with 20% Glycerol保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].SWISS:P08505Gene ID:16193产品图片
Recombinant human IL-22 protein产品编号bs-43572P英文名称Recombinant human IL-22 protein中文名称重组人白介素22蛋白性 状Lyophilized or Liquid浓 度1mg/ml保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍产品图片
Recombinant human IL-10 protein (Active, HEK293) 产品编号ck0006英文名称Recombinant human IL-10 protein (Active, HEK293)中文名称重组人白介素10 (活性的, Tag free)别 名Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. ;Recombinant human IL-10 protein (Active, HEK293) 人白介素10 重组蛋白; 人白细胞介素10 ; 重组人白介素10; 重组人IL-10;理论分子量17kDa检测分子量21 kDa性 状Lyophilized powder浓 度>0.5 mg/ml物 种Human序 列19-178/178纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素<0.5 EU/μg as determined by LAL.表达系统HEK293 cell活性Yes标签Tag free缓 冲 液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存条件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Interleukin 10(IL-10) is a member of the IL-10 family of α-helical cytokines. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of a number of proinflammatory cytokines such as IFNgamma, IL-2, IL-3, TNF and GM-CSF. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.This recombinant human IL-10 is produced by human cells.(Tag free)Biological activity:The activity was measured by its ability to stimulate the proliferation of MC/9-2 cells (mouse master cell line).Reconstitution:Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.SWISS:P18893Gene ID:3586产品图片
产品编号V0401英文名称human IL-6中文名称人白介素6单克隆抗体别 名Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; Interleukin-6; Interleukin6; IL6_HUMAN. 研究领域肿瘤 细胞生物 免疫学 神经生物学 生长因子和激素 抗体来源Mouse克隆类型Monoclonal克 隆 号6F5K交叉反应(predicted: Human, )产品应用ELISA=1:5000-10000 not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.理论分子量23kDa细胞定位分泌型蛋白 性 状Liquid浓 度≥1mg/ml免 疫 原Recombinant human IL-6 (C-6x His-Tag) 亚 型IgG1纯化方法affinity purified by Protein G缓 冲 液0.01M PBS(pH7.4) with 0.1% Proclin300.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.PubMedPubMed 产品介绍Interleukin 6 (IL-6), promptly and transiently produced in response to infections and tissue injuries, contributes to host defense through the stimulation of acute phase responses, hematopoiesis, and immune reactions.Function:Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.Subcellular Location:Secreted.Post-translational modifications:N- and O-glycosylated.DISEASE:Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.Similarity:Belongs to the IL-6 superfamily.SWISS:P05231Gene ID:3569Database links:Entrez Gene: 3569 HumanEntrez Gene: 16193 MouseEntrez Gene: 24498 RatOmim: 147620 HumanSwissProt: P05231 HumanSwissProt: P08505 MouseSwissProt: P20607 RatUnigene: 654458 HumanUnigene: 1019 MouseUnigene: 9873 Rat
Recombinant human IL-15 protein (Active, HEK293) 产品编号ck0013产品分类重组蛋白英文名称Recombinant human IL-15 protein (Active, HEK293)中文名称重组人白介素15 (活性的, Tag free)英文别名rhIL-15; Interleukin-15; IL 15; Interleukin 15; Interleukin15; MGC9721; IL15; IL15_HUMAN. ;Recombinant human IL-15 protein (Active, HEK293)中文别名人白介素15重组蛋白; 重组人白介素15; 人重组白介素15;纯化方法AC检测分子量14-15 kDaGeneID3600SwissP40933浓度>0.5 mg/ml储存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存条件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationIsoform IL15-S48AA: Secreted. Isoform IL15-S21AA: Cytoplasm. Nucleus. Note=IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.Tissue SpecificityMost abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.SimilarityBelongs to the IL-15/IL-21 family.Database linksEntrez Gene: 3600 HumanOmim: 600554 HumanSwissProt: P40933 HumanUnigene: 654378 Human多肽活性Yes蛋白质与多肽-多肽序列49-162/162小分子偶联蛋白-蛋白载体标签Tag free纯度>95% as determined by SDS-PAGE内毒素<0.5 EU/μg as determined by LAL.重组蛋白-表达系统HEK293 cell复溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
产品编号ck0017产品分类重组蛋白英文名称Recombinant human IL-2 protein (Active, HEK293)中文名称重组人白介素2 (活性的, Tag free)英文别名Interleukin-2; Interleukin 2; Interleukin2; rhIL-2; T-cell growth factor; T Cell Growth Factor; Aldesleukin; IL 2; IL2; Lymphokine; POIL2; T Cell Growth Factor; TCGF; Involved in regulation of T cell clonal expansion; IL2_HUMAN. ;Recombinant human IL-2 protein (Active, HEK293)中文别名人白介素2重组蛋白; 重组人白介素2; 人重组白介素2; 重组人白介素2(IL-2);蛋白细胞定位分泌型蛋白纯化方法AC理论分子量15kDa检测分子量17 kDaGeneID3558SwissP60568浓度>0.5 mg/ml储存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存条件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationSecreted.DISEASENote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.SimilarityBelongs to the IL-2 family.Database linksEntrez Gene: 3558 HumanEntrez Gene: 16183 MouseEntrez Gene: 116562 RatOmim: 147680 HumanSwissProt: P60568 HumanSwissProt: P04351 MouseSwissProt: P17108 RatUnigene: 89679 HumanUnigene: 14190 MouseUnigene: 9871 Rat背景资料The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]多肽活性Yes蛋白质与多肽-多肽序列21-153/153小分子偶联蛋白-蛋白载体标签Tag free纯度>95% as determined by SDS-PAGE内毒素<0.5 EU/μg as determined by LAL.重组蛋白-表达系统HEK293 cell复溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
产品编号bs-0295G-HRP英文名称Goat Anti-Rabbit IgG H&L / HRP中文名称辣根过氧化物酶标记的羊抗兔IgG H&L别 名Goat Anti-Rabbit IgG H&L (HRP); Immunoglobulin G; Specific References (703) | bs-0295G-HRP has been referenced in 703 publications.111 [IF=5.21] Ma, Yanqin, et al. "Inflammatory responses induced by fluoride and arsenic at toxic concentration in rabbit aorta." Archives of toxicology 86.6 (2012): 849-856. WB ; Rabbit. 222111 [IF=4.26] Wang, Ye-Jun, et al. "Two oral HBx vaccines delivered by live attenuated< i> Salmonella: Both eliciting effective anti-tumor immunity." Cancer letters 263.1 (2008): 67-76. WB ; Rabbit. 222111 [IF=4.26] Li, Gang, et al. "Overexpression of human papillomavirus (HPV) type 16 oncoproteins promotes angiogenesis< i> via enhancing HIF-1?? and VEGF expression in non-small cell lung cancer cells."Cancer letters 311.2 (2011): 160-170.. WB ; Rabbit. 222111 [IF=3.55] Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012). WB ; Rabbit. 222111 [IF=3.54] Zhang, Boce, Ariella Feldman, and Qin Wang. "A novel insight in rapid allergen detection in food systems: From threshold dose to real-world concentration."Sensors and Actuators B: Chemical?186 (2013): 597-602. ELISA ; Rabbit. 222111 [IF=3.06] Zhang, Zi?\ji, et al. "iRNA expression profile during osteogenic differentiation of human adipose?\derived stem cells."Journal of cellular biochemistry 113.3 (2012): 888-898..c WB ; Rabbit. 222111 [IF=3.06] Li, Guiying, et al. "Gremlin Aggravates Hyperglycemia?\Induced Podocyte Injury by a TGF??/Smad Dependent Signaling Pathway."ournal of cellular biochemistry?(2013).:: WB ; Rabbit. 222111 [IF=2.8] Jiang, An, et al. "miR-615-3p promotes the phagocytic capacity of splenic macrophages by targeting ligand-dependent nuclear receptor corepressor in cirrhosis-related portal hypertension." Experimental Biology and Medicine 236.6 (2011): 672-680. WB ; Rabbit. 222111 [IF=2.75] Dong, Xian-Zhe, et al. "A representative prescription for emotional disease, Ding-Zhi-Xiao-Wan restores 5-HT system deficit through interfering the synthesis and transshipment in chronic mild stress-induced depressive Rats." Journal of Ethnopharmacology ( WB ; Rabbit. 222111 [IF=2.7] Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013). WB ; Rabbit. 222111 [IF=2.7] WANG, Peng, et al. "Construction and identification of a recombinant adenovirus vector expressing His-tag-ICP47 fusion gene." Life Science Journal 9.1 (2012). Rabbit. 222111 [IF=2.58] Li, Yuping, et al. "Direct detection of rutin degrading isozymes with PAGE."Analytical Biochemistry (2013). WB ; Rabbit. 222PubMed:24050968111 [IF=2.41] Cong, Liang, et al. "Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic< i> hIGF-I silkworms." Biochemical and biophysical research communications 410.4 (2011): 721-725. WB ; Rabbit. 222111 [IF=2.07] Ma, Song‐Tao, et al. "Effect of Arctiin on Glomerular Filtration Barrier Damage in STZ‐Induced Diabetic Nephropathy Rats." Phytotherapy Research (2012). Rabbit. 222111 [IF=1.99] Fan, Tingjun, et al. "Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane." Molecular vision 17 (2011): 469. Rabbit. 222111 [IF=1.89] Yan, Shu, et al. "Expression of endoplasmic reticulum stress-related factors in the retinas of diabetic rats." Experimental diabetes research 2012 (2011). WB ; Rabbit. 222111 [IF=1.77] Chen, Cui-Ying, et al. "Characterization of cytotoxicity-related gene expression in response to virulent Marek’s disease virus infection in the bursa of Fabricius." Research in Veterinary Science (2012). WB ; Rabbit. 222111 [IF=1.74] Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013). WB ; Rabbit. 222111 [IF=.96] Yang, L., et al. "Transplantation of Schwann Cells Differentiated from Adipose-derived Stem Cells Modifies Reactive Gliosis after Contusion Brain Injury in Rats." Journal of International Medical Research 39.4 (2011): 1344-1357. WB ; Rabbit. 222111 [IF=.57] Lu, Shuang, et al. "Preparation and characterization of the polyclonal antibody against GAR domain of microtubule actin cross-linking factor 1 (MACF1)." Afr. J. Biotechnol 10 (2011): 2156-2162. ELISA ; Rabbit. 222111 [IF=.34] Feng, Yongqiang, et al. "Combination of ketamine and xylazine exacerbates cardiac dysfunction in severely scalded rats during the shock stage." Experimental and Therapeutic Medicine (2013). WB ; Rabbit. 222PubMed:4137240111 [IF=2.59] Zhang, Huizhen, et al. "Treatment with carnosine reduces hypoxia-ischemia brain damage in a neonatal rat model." European Journal of Pharmacology (2014). WB ; Rabbit. 222111 [IF=3.42] Xu, R., et al. "Secretoneurin Induces Airway Mucus Hypersecretion by Enhancing the Binding of EGF to NRP1." Cellular Physiology and Biochemistry 33.2 (2014): 446-456. WB ; Rabbit. 222111 [IF=3.01] Wang, Ying, et al. "Iso-suillin from the mushroom< i> Suillus flavus induces cell cycle arrest and apoptosis in K562 cell line." Food and Chemical Toxicology (2014). WB ; Rabbit. 222111 [IF=1.73] Zeng, Q. J., et al. "Decorin-induced proliferation of avian myoblasts involves the myostatin/Smad signaling pathway." Poultry Science 93.1 (2014): 138-146. WB ; Rabbit. 222111 [IF=3.73] Chen, Feng, et al. "Up-Regulating CYP3A4 Expression in C3A Cells by Transfection with a Novel Chimeric Regulator of hPXR-p53-AD." PLOS ONE 9.5 (2014): e95752. WB ; Rabbit. 222111 [IF=3.5] Zhang, Peng, et al. "In Situ Tracking the Intracellular Delivery of Antisense Oligonucleotides by Fluorescein Doped Silica Nanoparticles." Talanta (2014). WB ; Rabbit. 222111 [IF=7.6] Gao, Fuping, et al. "Cytotoxicity and therapeutic effect of irinotecan combined with selenium nanoparticles." Biomaterials (2014). IHC-P ; Rabbit. 222111 [IF=3.79] Luo, Xiaoming, et al. "Antimetastasis and antitumor efficacy promoted by sequential release of vascular disrupting and chemotherapeutic agents from electrospun fibers." International Journal of Pharmaceutics (2014). IHC-P ; Rabbit. 222111 [IF=3.39] Qiao, Hui, et al. "Progressive alterations of hippocampal CA3-CA1 synapses in an animal model of depression." Behavioural Brain Research (2014). WB ; Rabbit. 222抗体来源Goat克隆类型Polyclonal交叉反应Rabbit, 产品应用WB=1:10000-50000 ELISA=1:50000-200000 IHC-P=1:200 not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.性 状Liquid浓 度2.0 mg/ml免 疫 原Native rabbit IgG 亚 型IgG纯化方法affinity purified by Protein G, nonspecific adsorbed缓 冲 液10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.产品图片Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.Immobilized rabbit IgG (bs-0295P, nothing else) at 0.2μg/well can bind Goat Anti-Rabbit IgG H&L/HRP antibody (Cat. No. bs-0295G-HRP).
产品编号bs-41494P英文名称Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R), N-His中文名称重组SARS冠状病毒2核衣壳突变体蛋白Omicron(P13L、E31del、R32del、S33del、R203K、G204R)别 名Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R), N-His 理论分子量45.3kDa检测分子量50 kDa性 状Lyophilized or Liquid浓 度>0.5 mg/ml物 种SARS-Cov-2序 列1-419/419纯 度>90% as determined by SDS-PAGE纯化方法AC内毒素Not analyzed表达系统E.coli活性Not tested标签N-His缓 冲 液10mM PB (pH=7.4) with 50mM Nacl保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.SWISS:P0DTC9Gene ID:43740575产品图片The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.